Thermo Fisher Scientific ARFGAP1 Monoclonal Antibody (OTI 2C10), TrueMAB
Human ARFGAP1 단백질을 인식하는 Mouse IgG2b 단일클론 항체. Western Blot, ICC/IF, Flow Cytometry에 사용 가능. Lyophilized 형태로 제공되며, PBS buffer와 trehalose 포함. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI 2C10 |
| Immunogen | Full length human recombinant protein of human ARFGAP1 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of about 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
ArfGAP1 is a 406-amino-acid regulatory protein containing an Arf-GAP domain involved in activating the GTPase activity of ADP ribosylation factor (ARF).
It promotes hydrolysis of ARF1-bound GTP, facilitating dissociation of coat proteins from Golgi-derived membranes and vesicles, playing an essential role in membrane trafficking and vesicle transport.
Activity is stimulated by phosphoinositides and inhibited by phosphatidylcholine. Two transcript variants encoding different isoforms have been reported, with ubiquitous expression observed in most tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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