Thermo Fisher Scientific Guanylyl Cyclase alpha 1 Polyclonal Antibody
Guanylyl Cyclase alpha 1 단백질을 인식하는 Thermo Fisher Scientific의 토끼 폴리클로날 항체. Western blot, IHC, ICC, ELISA 등 다양한 응용에 적합. Protein A로 정제된 고순도 항체로, -20°C에서 보관. 연구용 전용 제품.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:200 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry / Immunofluorescence (ICC/IF) | 1:100–1:500 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic peptide derived from human Guanylyl Cyclase alpha 1 (amino acids 301–400) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01 M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Guanylate cyclases belong to the adenylyl cyclase class-4/guanylyl cyclase family. There are two forms of guanylate cyclase:
- A soluble form (GCS or sGC), which acts as a receptor for nitric oxide
- A membrane-bound receptor form (GC), which serves as a peptide hormone receptor
The GC-C protein consists of:
- An extracellular domain
- A single transmembrane domain
- A cytoplasmic region with a kinase-like domain and a catalytic domain
It exists as two glycosylated forms: a 130 kDa precursor in the endoplasmic reticulum and a 145 kDa form on the plasma membrane. Ligand binding to the extracellular domain promotes cGMP accumulation. GC-C serves as the receptor for heat-stable enterotoxins from pathogenic E. coli and binds guanylin and uroguanylin peptides that regulate renal function in response to salt load.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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