Thermo Fisher Scientific Acetyl-Histone H2A (Lys5) Polyclonal Antibody
Rabbit polyclonal antibody recognizing acetylated Histone H2A (Lys5). 검증된 응용: WB, IHC, ICC, ELISA. Human, Mouse, Rat 시료에 반응. Protein A 정제, 1 mg/mL 농도. 연구용으로 염색체 구조 및 히스톤 변형 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | Assay-dependent |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic acetylpeptide derived from human Histone H2A around acetylation site of Lys5 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Histones are basic nuclear proteins responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units called nucleosomes. The linker histone H1 interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher-order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is located in the large histone gene cluster on chromosome 6.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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