Thermo Fisher Scientific DNA-PK Monoclonal Antibody (2-B3)
DNA-PK 단백질을 인식하는 마우스 단클론 항체로, Western blot, IHC, ICC/IF에 사용 가능. 인간 및 마우스 시료에 반응하며, 단백질 A로 정제된 액상 형태. DNA 손상 반응 연구용으로 적합. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2-B3 |
| Immunogen | Recombinant protein within human DNA-PKcs/PRKDC aa 3900–4128 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4, with 40% glycerol, 0.2% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2911709 |
Product Specific Information
- Positive Control: HepG2, HeLa
- Subcellular Location: Nucleus
Target Information
DNA-PK is a heterotrimeric, DNA-dependent protein kinase that initiates signaling cascades in response to DNA damage. It is an atypical kinase closely related to PI3 kinases and can be inhibited by small molecules targeting PI3 kinases. DNA-PK encodes a component of the mediator complex, a transcriptional coactivator complex required for the expression of most genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, possibly by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. Multiple transcript variants encoding different isoforms have been identified for DNA-PK.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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