Thermo Fisher Scientific Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 680

Applications

Tested Dilution

Western Blot (WB)

1:5,000-1:20,000

Immunocytochemistry (ICC/IF)

1:500-1:2,000

Product Specifications

Species Reactivity

Rabbit

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Secondary Antibody

Immunogen

Gamma Immunoglobins Heavy and Light chains

Conjugate

Alexa Fluor™ 680 Alexa Fluor™ 680 Alexa Fluor™ 680

View additional formats

• [Alexa Fluor 350 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Highly Cross-Adsorbed/conjugates/Alexa Fluor 350/host/Goat/)
• [Alexa Fluor 488 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Highly Cross-Adsorbed/conjugates/Alexa Fluor 488/host/Goat/)
• [Alexa Fluor 546 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Highly Cross-Adsorbed/conjugates/Alexa Fluor 546/host/Goat/)
• [Alexa Fluor 555 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Highly Cross-Adsorbed/conjugates/Alexa Fluor 555/host/Goat/)
• [Alexa Fluor 568 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Highly Cross-Adsorbed/conjugates/Alexa Fluor 568/host/Goat/)
• [Alexa Fluor 594 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Highly Cross-Adsorbed/conjugates/Alexa Fluor 594/host/Goat/)
• [Alexa Fluor 633 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Highly Cross-Adsorbed/conjugates/Alexa Fluor 633/host/Goat/)
• [Alexa Fluor 635 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Highly Cross-Adsorbed/conjugates/Alexa Fluor 635/host/Goat/)
• [Alexa Fluor 647 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Highly Cross-Adsorbed/conjugates/Alexa Fluor 647/host/Goat/)
• [Alexa Fluor 660 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Highly Cross-Adsorbed/conjugates/Alexa Fluor 660/host/Goat/)
• [Alexa Fluor 790 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Highly Cross-Adsorbed/conjugates/Alexa Fluor 790/host/Goat/)
• [Alexa Fluor Plus 405 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Highly Cross-Adsorbed/conjugates/Alexa Fluor Plus 405/host/Goat/)
• [Alexa Fluor Plus 488 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Highly Cross-Adsorbed/conjugates/Alexa Fluor Plus 488/host/Goat/)
• [Alexa Fluor Plus 555 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Highly Cross-Adsorbed/conjugates/Alexa Fluor Plus 555/host/Goat/)
• [Alexa Fluor Plus 594 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Highly Cross-Adsorbed/conjugates/Alexa Fluor Plus 594/host/Goat/)
• [Alexa Fluor Plus 647 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Highly Cross-Adsorbed/conjugates/Alexa Fluor Plus 647/host/Goat/)
• [Alexa Fluor Plus 680 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Highly Cross-Adsorbed/conjugates/Alexa Fluor Plus 680/host/Goat/)
• [Alexa Fluor Plus 800 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Highly Cross-Adsorbed/conjugates/Alexa Fluor Plus 800/host/Goat/)
• [AP](/antibody/secondary/product-name/Rabbit IgG (H--L) Highly Cross-Adsorbed/conjugates/AP/host/Goat/)
• [Biotin ](/antibody/secondary/product-name/Rabbit IgG (H--L) Highly Cross-Adsorbed/conjugates/Biotin/host/Goat/)
• [FITC](/antibody/secondary/product-name/Rabbit IgG (H--L) Highly Cross-Adsorbed/conjugates/FITC/host/Goat/)
• [HRP](/antibody/secondary/product-name/Rabbit IgG (H--L) Highly Cross-Adsorbed/conjugates/HRP/host/Goat/)
• [Oregon Green 488](/antibody/secondary/product-name/Rabbit IgG (H--L) Highly Cross-Adsorbed/conjugates/Oregon Green 488/host/Goat/)
• [TRITC](/antibody/secondary/product-name/Rabbit IgG (H--L) Highly Cross-Adsorbed/conjugates/TRITC/host/Goat/)
• Request custom conjugation

Excitation/Emission Max

681/704 nm View spectra

Form

Liquid

Concentration

2 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.5

Contains

5mM sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient

RRID

AB_2535758

Target

IgG

Cross Adsorption

Against bovine IgG, goat IgG, mouse IgG, rat IgG and human IgG

Antibody Form

Whole Antibody

Product Specific Information

To minimize cross-reactivity, these goat anti-rabbit IgG (H+L) whole secondary antibodies have been affinity purified and cross-adsorbed against bovine IgG, goat IgG, mouse IgG, rat IgG, and human IgG. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in higher sensitivity and less background staining. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. The benefits of this extra step are apparent in multiplexing/multicolor-staining experiments (e.g., flow cytometry) where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there are may be the presence of endogenous immunoglobulins.

Alexa Fluor dyes are among the most trusted fluorescent dyes available today. Invitrogen™ Alexa Fluor 680 dye is a bright, near-infrared-fluorescent dye with excitation ideally suited to the 680 nm laser line. For stable signal generation in imaging and flow cytometry, Alexa Fluor 680 dye is pH-insensitive over a wide molar range. Probes with high fluorescence quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity. Alexa Fluor 680 dye molecules can be attached to proteins at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection. The degree of labeling for each conjugate is typically 2-8 fluorophore molecules per IgG molecule; the exact degree of labeling is indicated on the certificate of analysis for each product lot.

Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically. For the fluorophore-labeled antibodies a final concentration of 1-10 µg/mL should be satisfactory for most immunohistochemistry and flow cytometry applications, and 0.04-0.2 µg/mL for western blotting.

Product will be shipped at Room Temperature.

Target Information

Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.