Invitrogen™

SuperScript™ III Platinum™ SYBR™ Green One-Step qPCR Kit w/ROX

Our One-Step qRT-PCR Kits with ROX combine the most powerful reverse transcriptase and DNA polymerase technologies to deliver precise and자세히 알아보기

Our One-Step qRT-PCR Kits with ROX combine the most powerful reverse transcriptase and DNA polymerase technologies to deliver precise and accurate analysis of gene expression in a convenient, high-throughput one-step format. One-Step qRT-PCR kits with ROX include:

–SuperScript™ III Reverse Transcriptase: produces higher yields of full-length cDNA for broader gene representation

–Platinum™ Taq DNA Polymerase: activates faster and more completely than other hot-start enzymes to deliver more sensitive amplification (Figure 1)
–ROX reference dye premixed at optimal concentration for ABI instruments

Two kit configurations allow your choice of detection methods:

–SuperScript™ III Platinum™ SYBR™ Green One-Step qRT-PCR Kit provides a highly efficient, cost-effective qRT-PCR system with SYBR Green I detection
–SuperScript™ III Platinum™ One-Step qRT-PCR Kit provides sensitive, specific detection for use with LUX™ fluorogenic primers or dual-labeled probes (e.g. TaqMan™) (Figure 2)

Contents and Storage:
SuperScript™ III Platinum™ SYBR™ Green One-Step qRT-PCR Kit with ROX includes SuperScript™ III RT/Platinum™ Taq Mix, 2X Reaction Mix (containing SYBR™ Green I, ROX Reference Dye, dNTPs, and MgSO4), and 50 mM MgSO4. SuperScript™ III Platinum™ One-Step qRT-PCR Kit with ROX includes SuperScript™ III RT/Platinum™ Taq Mix, 2X Reaction Mix (containing ROX Reference Dye, dNTPs and MgSO4), and 50 mM MgSO4. Store all components at -20°C. Store ROX Reference dye in the dark. Guaranteed stable for 6 months when properly stored.

사양

검출 방법SYBR

PCR 방법1-step RT-qPCR

중합효소Taq DNA Polymerase

반응 속도Standard

용도(장비)7000 System, 7300 System, 7700 System, 7900HT System

제품라인Platinum™, SYBR™, SuperScript™

제품 유형Real Time PCR SYBR Master Mix

수량100 reactions

배송 조건Dry Ice

충분100 Reactions

용도(애플리케이션)Gene Expression

농도2X

반응 수100 Reactions

샘플 종류RNA

Unit SizeEach