Thermo Fisher Scientific RNASEH2A Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:50-1:400

-

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:100

-

Immunocytochemistry (ICC/IF)

1:50-1:500

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein Ribonuclease H2 Subunit A. The antigen corresponds to amino acid range 1-299 of the target protein. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:RNAse H2A,uniProtId:O75792-1,ncbiNodeId:9606,antigenRange:1-299,antigenLength:299,antigenImageFileName:PA5-119399_RNAse_H2A_O75792-1_Rabbit.svg,antigenImageFileNamePDP:PA5-119399_RNAse_H2A_O75792-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 50% glycerol

Contains

0.05% ProClin 300

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2903897

Target Information

Ribonucleases (RNAses) H are enzymes that hydrolyze the RNA strands of RNA/DNA hybrids. The major role of these enzymes is to remove the RNA strand from the RNA/DNA hybrids that form during DNA replication and repair. RNAse H2 is made up of three subunits; all three are required for RNAse activity. Recent evidence has demonstrated that mutations in RNAse H2A or any of the other subunits result in Aicardi-Goutieres syndrome (AGS), a neurological disorder with similar symptoms to viral brain infections including high levels of IFN-alpha in the cerebral spinal fluid. Similar conditions are observed with mutations in TREX1, a single-stranded DNA exonuclease, suggesting that RNAse H2 and TREX1 may have similar roles, and that mutations in any of these genes lead to an accumulation of intracellular nucleic acids, triggering an inflammatory response through activation of the innate immune system.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.