Thermo Fisher Scientific Cytokeratin 20 (KRT20) Monoclonal Antibody (KRT20, 1993)
인간 KRT20 단백질을 인식하는 마우스 단클론 항체로, Western blot 및 IHC에 적합. 비결합형 액상 형태이며 PBS buffer에 보존. 단백질 A/G로 정제되어 높은 특이성과 재현성 제공. 연구용으로만 사용 가능.
- 판매단위
- pk
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Applications
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1–2 µg/mL | |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL | |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | KRT20, 1993 |
| Immunogen | Recombinant fragment of human KRT20 protein (around aa 196–323) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): incubate antibody for 30 min at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, followed by cooling at room temperature for 20 min.
Target Information
The protein encoded by this gene is a member of the keratin family. Keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins.
Type I cytokeratins consist of acidic proteins arranged in pairs of heterotypic keratin chains.
Cytokeratin 20 is a major cellular protein of mature enterocytes and goblet cells, specifically expressed in the gastric and intestinal mucosa.
Type I cytokeratin genes are clustered in a region of chromosome 17q12–q21.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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