Thermo Fisher Scientific CD44/HCAM Std Monoclonal Antibody (BU75)
인간 및 비인간 영장류 반응성을 가진 CD44/HCAM 단클론 항체(BU75). 다양한 어플리케이션(WB, IHC, ICC/IF, Flow Cytometry)에 사용 가능. Protein A/G로 정제된 액상 형태, 200 µg/mL 농도. 세포 접착 및 이동 연구에 적합.
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Thermo Fisher Scientific CD44/HCAM Std Monoclonal Antibody (BU75)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 0.25–0.5 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
| Functional Assay (Functional) | Assay-dependent |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Non-human primate |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | BU75 |
| Immunogen | Follicular dendritic cells and germinal B cells |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
CD44 cell surface antigen is a 100 kDa type I transmembrane glycoprotein widely expressed on human leukocytes, white matter of the brain, and some epithelial cells of the intestine and breast. Several isoforms of CD44 exist, including the predominant CD44H isoform detected in many normal tissues.
CD44 functions as a receptor for hyaluronic acid (HA) and plays roles in cell-cell interactions, adhesion, and migration. It participates in lymphocyte activation, recirculation, and homing. Expression may be up-regulated in certain carcinomas, potentially relating to metastatic potential.
CD44 is expressed by hematopoietic and non-hematopoietic cells, epithelial tissues, and filopodia in cultured keratinocytes. Bone marrow myeloid cells and memory T cells express CD44 at high levels, while peripheral B and T cells can upregulate CD44 in response to stimulation.
Transcripts for the CD44 gene undergo complex alternative splicing, generating many functionally distinct isoforms. This splicing contributes to the structural and functional diversity of CD44.
Diseases associated with CD44 dysfunction include superficial keratitis and lichen sclerosus. CD44 may also be implicated in tumor metastasis formation.
Important Notice
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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