Invitrogen™

One Shot™ Stbl3™ Chemically Competent E. coli

One Shot Stbl3 Chemically Competent E. coli are designed for cloning direct repeats found in lentiviral expression vectors as these자세히 알아보기

One Shot Stbl3 Chemically Competent E. coli are designed for cloning direct repeats found in lentiviral expression vectors as these cells reduce the frequency of homologous recombination of direct repeats found in lentiviral vectors (ViraPower Lentiviral Expression kits) and other retroviral vectors (instability-prone HIV-based plasmids). The Stbl3 strain has been essential for many applications, including genome editing CRISPR workflows, vaccine development (including SARS-CoV-2 vaccine research), cloning, and maintaining vectors with multiple repeats (e.g., gene coding protein with repeated sequence). The transformation efficiency of One Shot Stbl3 chemically competent cells is >1 x 108 cfu/μg plasmid DNA.

The Stbl3 strain is derived from the HB101 E. coli strain with a hybrid K-12 and B strain genome. It contains the _rec_A13 allele of the _rec_A mutation that reduces nonspecific recombination occurrence in cloned DNA. Mutations in the Stbl3 methylation-dependent restriction system (_mcr_B, _mrr, _and _hsd_S20) allow construction of cloning genomic DNA. This strain has _end_A1+, a nonspecific endonuclease I that digests DNA. The Stbl3 strain also reproducibly yields more plasmid DNA than DH strains (10-fold or more plamsmid DNA than the TOP10 strain) and sustainably produces high titer lentivirus.

One Shot Stbl3 Chemically Competent E. coli offer:
• Expected efficiency of >1 x 108 transformants/μg plasmid DNA
• Optimal performance for the propagation of lentiviral vectors and maintenance of direct repeats
• Enhanced genomic DNA cloning capacity (mcr_B, _mrr, and _hsd_S20)
• Capable of producing high DNA yields

Note: For optimal performance and to maintain plasmid with complex repeat sequences, Stbl3 cells should be cultured at 30°C. When performing plasmid DNA isolation from Stbl3 cells that have wild type endonuclease I (end_A1+), ensure that buffers contain 10 mM EDTA. EDTA will inactivate the endonuclease and avoid DNA nicking and vector degradation (follow plasmid DNA purification kit instructions for _end_A1+ _E. coli strains).

Easy-to-use One Shot format
Stbl3 Chemically Competent E. coli cells are supplied in the convenient, single-reaction One Shot format. The single-tube, single-use format allows all steps of the transformation protocol, up to plating, to take place in the same tube, thereby helping save time and prevent contamination.

Genotype
F–_mcr_B _mrr hsd_S20(rB–, mB–) _rec_A13 _sup_E44 _ara_-14 _gal_K2 _lac_Y1 _pro_A2 _rps_L20(StrR) _xyl_-5 λ–_leu mtl_-1

Find the strain and format that fit your needs
Other Stbl strains are available with different genetic background.
The Stbl3 strain is available in MultiShot format for high throughput applications.
Explore bacterial growth media formats

사양

제품 유형Chemically Competent Cells

에프에피솜 포함No

플라스미드 품질 개선No

메틸화 DNA 클로닝Yes (mcrB, mrr)

형질전환 효율 수준Medium Efficiency (1 x 108 to 1 x 109 cfu/μg)

항생제 내성 박테리아(Yes) Streptomycin

불안정 DNA 클로닝Yes (recA13)

블루/화이트 스크리닝No

고처리량 호환성Low

플라스미드May be used for plasmids >20 kb

비메틸화 DNA 준비No

재조합 감소Yes (recA13)

배송 조건Dry Ice

T1 Phage - 저항성(tonA)No

종E. coli (K12/B hybrid)

형식Tube

제품라인One Shot™

수량21 x 50 μL/tube

Unit SizeEach