Gibco™

FreeStyle™ F17 Expression Medium

Gibco FreeStyle F17 Expression Medium is a chemically defined, protein-free, serum-free medium developed to support the growth and transfection of자세히 알아보기

Gibco FreeStyle F17 Expression Medium is a chemically defined, protein-free, serum-free medium developed to support the growth and transfection of HEK293 cells in suspension. The addition of 4–8 mM L-glutamine or GlutaMAX supplement is required before use.

Features:
• Little or no required adaptation
• Supports suspension growth and transfection of HEK293 cells
• Chemically defined, protein-free, serum-free formulation
• Compatible with multiple transient production systems
• Scalability in spinner flasks and bioreactors

Little or no required adaptation
293-F, 293-H, and FreeStyle 293-F cells in suspension can be cultured directly into FreeStyle F17 Expression Medium without adaptation. Cells adapted to other commercially available serum-free media can be subcultured directly into FreeStyle F17 medium, usually without any further adaptation. Cells usually require adaptation from serum-containing formulations.

Growth and transfection of HEK293 cells
FreeStyle F17 Expression Medium supports 293-F cells at a peak density of 6–7 x 106 viable cells per mL. Cells cultured in FreeStyle F17 medium can be transfected using a wide variety of transfection reagents, including lipid-based transfection reagents, such as 293fectin and Freestyle MAX Reagent.

Scalability in spinner flasks and bioreactors
Production using FreeStyle F17 Expression Medium can be scaled up in spinner flasks or bioreactors. The appropriate spinner or impeller speed and seeding density should be optimized for each system. Depending on the impeller design and speed, it may be necessary to supplement FreeStyle F17 medium with additional Pluronic F-68 to avoid sheer stress in the culture.

cGMP manufacturing and quality system
FreeStyle F17 Expression Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Design your own medium specific to your needs
Whether it`s a bench-level experiment or large-scale biomanufacturing process, we offer specialized formats, packaging, and quality levels specific to your needs. With Gibco cGMP Media Custom Services you can put the manufacturing power and years of technical experience to work for you.

사양

농도 비율1:1

배양 환경CO2

세포주HEK293

분류Animal Origin-free, Chemically-defined, Protein-free, Serum-free

농도1 X

Culture TypeSuspension Cell Culture

형태Liquid

제품 유형Expression Medium

Serum LevelSerum-free

유통 기한12 Months

멸균Sterile

첨가제 없음No Glutamine

엔도톡신 수준< 1 EU/mL

형식Bottle

용도(애플리케이션)Protein expression, viral vector production

제품라인Freestyle™

순도 또는 품질 등급Qualified for Further Manufacturing

수량1 L

배송 조건Room Temperature

Unit SizeEach