Invitrogen™

pEXP4-DEST Vector Kit

Expressway™ Tag-On-Demand™ Cell-Free E. coliExpression System with Lumio™ Technology enables convenient, in vitro expression of tagged or untagged protein from자세히 알아보기

Expressway™ Tag-On-Demand™ Cell-Free E. coli_Expression System with Lumio™ Technology enables convenient, _in vitro expression of tagged or untagged protein from a single vector, real-time detection of protein synthesis, and easy in-gel protein detection. With this system, you can:

• Quickly screen for expression levels of full-length protein
• Save time and effort by cloning only once to express tagged or native protein
• Express a C-terminally tagged protein from an Ultimate™ ORF Clone in an in vitro system

The Expressway™ Tag-On-Demand™ System includes a specialized _E. coli_lysate derived from a _sl_yD mutant for cell-free synthesis. This lysate eliminates non-specific binding of the Lumio™ Green Detection Reagent to the endogenous SlyD protein, providing an optimal background for recombinant protein detection.

*How it works
*Simply clone your gene of interest containing a TAG (amber) stop codon into the provided pEXP4-DEST (Figure 1). The pEXP4-DEST vector features _at_tR sites for efficient Gateway™ recombination, C-terminal Lumio™ and 6xHis tags for simplified identification and purification, and components for cell-free protein synthesis. In the presence of the Expressway™ Tag-On-Demand™ Suppressor tRNA, the TAG codon is not recognized and translation continues through the Lumio™ and 6xHis tags. In the absence of the Suppressor tRNA, the TAG stop codon is recognized and native protein is produced (Figure 2). From the same vector, you can rapidly determine the expression level of the C-terminal-tagged, full-length protein, and then express the native protein free from any adverse affects the tag may have on its structure, activity, or molecular interactions.

사양

프로모터T7

제품 유형Gateway System Destination Expression Vector

선택 제제(진핵)None

항생제 내성 박테리아Ampicillin (AmpR), Zeocin™ (ZeoR)

단백질 태그Lumio

클로닝 방법Gateway

수량6 μg

벡터pEXP, pDEST

제품라인Expressway™, Gateway™

Unit Size6 µg