Thermo Fisher Scientific ERCC1 Monoclonal Antibody (OTI5E2), TrueMAB
ERCC1 단백질을 인식하는 mouse monoclonal antibody로, Western blot과 IHC(P)에서 검증됨. 인간 시료에 반응하며, 고순도 affinity chromatography로 정제됨. 무보존제, 동결건조 형태로 연구용 DNA 복구 관련 연구에 적합.
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Applications
Western Blot (WB)
- Tested Dilution: 1:2,000
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:150
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI5E2 |
| Immunogen | Full length human recombinant protein of human ERCC1 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
ERCC1 is a non-catalytic component of a structure-specific DNA repair endonuclease responsible for the 5′-incision during DNA repair.
It works with SLX4 in the first step of interstrand cross-link (ICL) repair and participates in processing anaphase bridge-generating DNA structures that may cause cytokinesis failure.
ERCC1 is also required for homology-directed repair (HDR) of DNA double-strand breaks in conjunction with SLX4. (Uniprot)
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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