Himedia Nutrient Mixture F-12 Ham, Coon`s Modification w/ L-Glutamine w/o Sodium bicarbonate

Himedia Nutrient Mixture F-12 Ham, Coon's Modification w/ L-Glutamine w/o Sodium Bicarbonate

Ham’s Nutrient Mixtures were originally developed for single-cell plating of near diploid Chinese hamster ovary (CHO) cells and mouse L-cells.
F-12 is formulated for use with or without serum, depending on the type of cells being cultured.

Ham’s Nutrient Mixture F-12, Coon’s modification was developed to determine optimal conditions for obtaining viable hybrids by treatment of parental cells with Sendai virus.
This medium was modified by doubling the concentration of amino acids and pyruvate and by adding ascorbic acid to enhance cell viability.

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