Thermo Fisher Scientific NeuN Polyclonal Antibody
Rabbit polyclonal antibody against human NeuN (Fox-3) for WB, IHC, and ICC applications. High specificity and purity via antigen affinity chromatography. Supplied as liquid form, unconjugated, with PBS/glycerol buffer. Ideal for neuronal marker detecti...
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:500 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human NeuN. Recombinant protein control fragment (Product #RP-93368). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.10 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2644627 |
Product Specific Information
Immunogen sequence:
PTQDYSGQTP VPTEHGMTLY TPAQTHPEQP GSEASTQPIA GTQTVPQTDE AAQTDSQPLH PSDPTEKQQ
Ortholog Sequence Identity:
- Mouse: 93%
- Rat: 94%
Target Information
Fox-3, also known as NeuN, HRNBP3, RBFOX3 (RNA binding protein, fox-1) or Fox-1 homolog C, is a 350 amino acid protein containing one RRM (RNA recognition motif) domain. It is localized to both the nucleus and cytoplasm and is suggested to regulate alternative splicing events.
Fox-3 is encoded by a gene located on human chromosome 17, which comprises over 2.5% of the human genome and encodes more than 1,200 genes. Two key tumor suppressor genes are associated with chromosome 17 — p53 and BRCA1:
- p53: Maintains cellular genetic integrity through DNA repair and cell fate regulation. Loss or malfunction is linked to malignant growth and Li-Fraumeni syndrome.
- BRCA1: Involved in DNA repair and recognized as a genetic determinant of early-onset breast cancer and predisposition to ovarian, colon, prostate, and fallopian tube cancers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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