
Thermo Fisher Scientific Rabbit anti-Goat IgG Recombinant Secondary Antibody, Alexa Fluor Plus 647
Alexa Fluor Plus 647 결합형 Rabbit anti-Goat IgG 재조합 2차 항체로 높은 신호 대비와 낮은 배경을 제공합니다. WB, ICC/IF, Flow Cytometry에 적합하며, Fc 수용체 결합이 제거되어 일관된 결과를 제공합니다. 4°C에서 보관하며 동결 금지.
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Thermo Fisher Scientific Rabbit anti-Goat IgG Recombinant Secondary Antibody, Alexa Fluor Plus 647
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 0.5–4 µg/mL |
| Flow Cytometry (Flow) | 1 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Goat |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Secondary Antibody |
| Clone | 21H44L11 |
| Conjugate | Alexa Fluor™ Plus 647 |
| Excitation / Emission Max | 658 / 675 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer, pH 6.5 |
| Contains | 0.008% Bromonitrodioxane, 0.008% Methylisothiazolone |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Wet ice |
| RRID | AB_2930909 |
| Target | IgG |
| Antibody Form | Whole Antibody |
Product Specific Information
Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include better specificity and superior lot-to-lot consistency.
This recombinant antibody has been engineered with point mutations that eliminate binding to Fc receptors, enabling reduction in background and superior signal-to-noise.
Alexa Fluor Plus secondary antibodies provide brighter signal compared to leading Alexa Fluor secondary antibodies, offering better signal-to-noise for critical experiments. They are conjugated using proprietary dye chemistry to generate vivid fluorescence images.
Usage Tip:
Centrifuge the conjugate solution briefly before use and use only the supernatant to minimize nonspecific background staining. Optimal dilution should be determined empirically for each application.
Target Information
Anti-Goat secondary antibodies are affinity-purified antibodies with well-characterized specificity for goat immunoglobulins. They are useful for detection, sorting, or purification of the specified target. Secondary antibodies enhance versatility and sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
These antibodies are typically generated by immunizing the host animal with pooled goat immunoglobulins and can be further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) for high specificity.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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