Thermo Fisher Scientific NovaBright Phospha-Light EXP Assay Kit for SEAP (Secreted Placental Alkaline Phosphatase) Reporter Gene Detection, 2 x 96 well plates, 192 assays

Invitrogen™ NovaBright™ Phospha-Light™ EXP Assay Kit for SEAP (Secreted Placental Alkaline Phosphatase) Reporter Gene Detection

The NovaBright™ Phospha-Light™ EXP Assay Kit is a second-generation chemiluminescent reporter gene assay for the ultrasensitive detection of secreted placental alkaline phosphatase (SEAP) or the non-secreted placental isozyme PLAP.
This assay combines high sensitivity with a dynamic range of five orders of magnitude, enabling superior performance compared to colorimetric or fluorescent detection methods.

주요 특징

• High-performance alkaline phosphatase substrate CSPD™ 사용
• Proprietary chemiluminescence enhancer 및 buffer system 포함
• Endogenous non-placental alkaline phosphatase activity 억제
• 두 가지 ready-to-use solution만으로 간단한 실험 절차
• Prolonged glow kinetics로 안정적인 신호 유지
• Injector 없는 단순한 luminometer로 측정 가능
• SEAP는 배양액으로 분비되어 세포 용해 불필요
• PLAP isoenzyme 검출도 가능 (cell lysis 후)

응용 분야

• Gene expression 분석 및 transfection 효율 최적화
• Viral function, replication, neutralization, infectivity assay
• Vaccine 개발 및 gene delivery 연구
• In vivo reporter gene assay (transgenic 또는 viral vector-infected 동물의 혈청 분석)
• Receptor-ligand binding assay 및 secretion pathway activity 분석
• Non-placental alkaline phosphatase biomarker 측정

사양

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