Thermo Fisher Scientific CD355 (CRTAM) Monoclonal Antibody (Cr24.1), APC, eBioscience

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (1 µg)/test

-

Product Specifications

Host/Isotype

Mouse / IgG2a, kappa

Recommended Isotype Control

Mouse IgG2a kappa Isotype Control (eBM2a), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

Cr24.1

Immunogen

Transfected CRTAM-P815 cells

Conjugate

APC APC APC

View additional formats

• Alexa Fluor 488
• PE
• PE-Cyanine7
• Request custom conjugation

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Wet ice

RRID

AB_2637276

Product Specific Information

This monoclonal antibody Cr24.1 reacts with human CD355, also known as Class-I MHC-restricted T-cell-associated molecule (CRTAM). CD355 usually exists as a homodimer, however, it forms a heterodimer with its ligand Necl-2. CD355 is transiently expressed by activated NK, NKT, gamma delta T, and CD8+ T cells. CD355 is also expressed by Purkinje neurons. Human CD355 is involved in immunosurveillance of cancer cells, gamma delta T cell survival, cell polarity, retention of lymphocytes in lymph nodes, promotion of cytotoxicity in NK cells, and IFN gamma secretion from CD8+ T cells. Cr24.1 has been reported to have antagonist activity.

This Cr24.1 antibody has been pre-titrated and tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The CRTAM gene is upregulated in CD4-positive and CD8-positive T cells and encodes a type I transmembrane protein with V and C1-like Ig domains.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.