Thermo Scientific™

NHS-Azide

Thermo Scientific NHS-Azide is an amine-reactive compound that can be used to derivatize primary amines of proteins or amine-coated polymer자세히 알아보기

Thermo Scientific NHS-Azide is an amine-reactive compound that can be used to derivatize primary amines of proteins or amine-coated polymer surfaces for ligation to phosphine-modified molecules.

This NHS-ester compound reacts to form covalent bonds with primary amines (e.g., side chain of lysine residues or aminosilane-coated surfaces). The azide (N3) group reacts with phosphine-labeled molecules by a mechanism known as Staudinger chemistry, enabling efficient and specific conjugation of derivatized molecules in biological samples.

Features of NHS-Azide:

• Soluble—reagent easily dissolves in water-miscible solvents for subsequent dilution in aqueous reaction mixtures with cell lysates and other biological samples
• Compatible—reaction chemistry occurs effectively in simple buffer conditions; requires no accessory reagents such as copper or reducing agents
• Specific—NHS esters are specific for covalent attachment to primary amines (side chain of lysine or N-terminus of polypeptides)
• Chemoselective—azide and phosphine groups do not react or interfere with components of biological samples (bioorthogonal) but conjugate to one another with high efficiency

Related Products
NHS-PEG4-Azide
NHS-Phosphine

사양

화학물질 반응성Amine-Phosphine

CleavableNo

설명NHS-Azide

분자량198.14

페길레이티드No

스페이서 암 길이2.5 Å

세포 투과성Not determined

배송 조건Ambient

제품라인Pierce™

라벨링 방법Chemical Labeling

교차결합제 유형Heterobifunctional

반응성 부분Amine, Azide, NHS Ester

스페이서Short (<10 Å)

형태Powder

수량10 mg

용해도DMF, DMSO

형식Standard

수용성No

제품 유형Crosslinker

Unit SizeEach