Thermo Fisher Scientific Thioredoxin 1 Recombinant Rabbit Monoclonal Antibody (1O2H10)
Thioredoxin 1 단백질을 인식하는 토끼 단클론 항체로, Western blot, IHC, ICC/IF, ELISA에 적합합니다. HEK293 발현 시스템에서 생산된 재조합 항체이며, 높은 특이성과 재현성을 제공합니다. 인간, 생쥐, 랫트 시료에 반응합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:6,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:2,000–1:8,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 1O2H10 |
| Immunogen | Synthetic peptide corresponding to amino acids 26–105 of human Thioredoxin 1 (Trx1/TXN, P10599) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.25 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 0.05% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2898020 |
Product Specific Information
- Positive Samples: HeLa, HepG2, MCF7
- Immunogen Sequence:
DFSATWCGPC KMIKPFFHSL SEKYSNVIFL EVDVDDCQDV ASECEVKCMP TFQFFKKGQK VGEFSGANKE KLEATINELV
Target Information
Thioredoxins (Trx) are small, multifunctional proteins with oxidoreductase activity and are present in virtually all living cells. Trx contains a redox-active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. The two cysteine residues in the conserved active centers can be oxidized to form intramolecular disulfide bonds. Reduction of the active site disulfide in oxidized Trx is catalyzed by Trx reductase with NADPH as the electron donor.
Reduced Trx acts as a hydrogen donor for ribonucleotide reductase, an essential enzyme for DNA synthesis, and serves as a general protein disulfide reductase involved in redox regulation. Specific targets include protein disulfide-isomerase (PDI) and transcription factors such as p53, NF-κB, and AP-1. Trx also contributes to hydrogen peroxide removal, particularly in conjunction with methionine sulfoxide reductase or peroxiredoxins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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