Thermo Fisher Scientific P4HA2 Polyclonal Antibody
P4HA2 단백질을 인식하는 Thermo Fisher Scientific의 토끼 폴리클로날 항체. Western blot, IHC(P), ICC/IF 등 다양한 응용에 적합. 항원 친화 크로마토그래피로 정제되었으며, 인간에 반응. 콜라겐 합성 관련 연구에 유용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:500 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human P4HA2. Recombinant protein control fragment (Product #RP-89681). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2645148 |
Product Specific Information
Immunogen sequence:
LKEYILVEEA KLSKIKSWAN KMEALTSKSA ADAEGYLAHP VNAYKLVKRL NTDWPALEDL VLQDSAAGFI ANLSVQRQFF PTDEDEIGAA KALMRLQDTY RLDPGTISRG ELPGTKYQAM LSVDDCFGM
Sequence identity:
Highest antigen sequence identity to the following orthologs:
- Mouse: 90%
- Rat: 91%
Target Information
This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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