Thermo Fisher Scientific Phospho-PDPK1 (Ser241) Monoclonal Antibody (K.424.2)
Phospho-PDPK1 (Ser241) 단백질을 특이적으로 인식하는 rabbit monoclonal 항체. Western blot 및 immunoprecipitation에 적합. Human, mouse, rat 반응성. 액상 형태로 보관하며, -20°C에서 안정적 저장 가능. 연구용 전용 제품.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunoprecipitation (IP) | 1:50 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | K.424.2 |
| Immunogen | Synthetic phosphopeptide corresponding to residues around pSer241 of human PDK1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 62 µg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100 µg/mL BSA, 50% glycerol |
| Contains | <0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_10978129 |
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
PDPK1 (3-Phosphoinositide Dependent Protein Kinase 1) phosphorylates AGC kinases and activates conventional PKC and PKC zeta through phosphorylation of critical threonine residues in the activation loop. It also phosphorylates Protein Kinase B (PKB) at threonine 308 in the presence of phosphatidylinositol-3,4,5-trisphosphate. Active Akt inactivates Glycogen Synthase Kinase 3 (GSK3), leading to activation of glycogen synthase and stimulation of glycogen synthesis. Due to its role in insulin-induced glycogen synthesis and PKC activation, PDPK1 is an important target for metabolic drug research.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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