Gibco™

MEM α, nucleosides, powder

Minimum Essential Medium (MEM) α is widely used for mammalian cell culture as well as selection for transfected DHFR negative자세히 알아보기

Minimum Essential Medium (MEM) α is widely used for mammalian cell culture as well as selection for transfected DHFR negative cells. MEM α can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells. We offer a variety of Gibco™ MEM α modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This MEM α is modified as follows:

With	Without
• Ribonucleosides	• Sodium Bicarbonate
• Deoxyribonucleosides
• L-glutamine
• Phenol Red

The complete formulation is available.

Gibco™ MEM α is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, B12, biotin, and ascorbic acid. MEM α is available without nucleosides for use as a selection medium for DG44 and other DHFR negative cells. This product is made with Earle`s salts.

MEM α contains no proteins, lipids, or growth factors. Therefore, MEM α requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM α uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

Powder forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).

사양

배양 환경CO2

세포 유형Mammalian Cells

분류Animal Origin-free

Culture TypeMammalian Cell Culture

형태Powder

제품 유형Minimum Essential Medium (MEM) alpha

Serum LevelStandard Serum Supplementation

유통 기한36 Months

첨가제 포함Glutamine, Phenol Red, Sodium Pyruvate

첨가제 없음No HEPES, No Sodium Bicarbonate

제조 품질cGMP-compliant under the ISO 13485 standard

삼투압240 - 290 mOsm/kg

pH3.3 to 3.9

제품라인Gibco™

수량1 L

배송 조건Room Temperature

Unit SizeEach