Thermo Fisher Scientific Human ABL1 [F317I], GST Tag Recombinant Protein

Applications

Tested Dilution

Publications

Kinase Assay (KA)

Assay-dependent

-

Product Specifications

Species

Human

Expression System

Insect cells

Amino acid sequence

27-end

Tag

GST-tag

Molecular weight

146.3 kDa

Class

Recombinant

Type

Protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Purification

purified

Storage buffer

50mM tris HCl, pH 7.5, with 25% glycerol, 0.25mM DTT, 150mM NaCl, 0.1mM PMSF, 0.1mM EDTA, 10mM glutathione

Contains

no preservative

Storage conditions

-80° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Dry ice

Product Specific Information

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 µg, 10 µg and 20 µg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 µL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase.

This ABL1 F317I recombinant human protein (27-end) was expressed in insect cells.

The purified catalytic domain (27-end) of the ABL1 F317I mutant can be used in a number of different applications, but has been specifically validated for use with our Z`-LYTE™ assay technology to identify novel kinase inhibitors. ABL1 F317I recombinant human protein is also available in our SelectScreen™ Kinase Profiling Service.

• Validated for use with the Z`-LYTE™ assay
• Available in multiple pack sizes for convenient use
• Also available in our SelectScreen Kinase Profiling Service

Protein Form: Recombinant, Active, Mutant

The gene accession number for ABL1 [F317I] is NP_005148.2.

Target Information

ABL1 cytoplasmic tyrosine kinase protooncogene encodes a cytoplasmic and nuclear kinase that has been implicated in processes of cell differentiation, cell division, cell adhesion, and stress response. Activity of ABL protein is negatively regulated by its SH3 domain and deletion of the SH3 domain turns ABL1 into an oncogene. Translocation and head-to-tail fusion of the BCR and ABL1 genes is present in many cases of chronic myelogeneous leukemia. The DNA-binding activity of the ubiquitously expressed ABL1 tyrosine kinase is regulated by CDK1-mediated phosphorylation, suggesting a cell cycle function for ABL1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.