Thermo Fisher Scientific CK1 gamma-1 Polyclonal Antibody
Rabbit polyclonal antibody targeting human CK1 gamma-1 (CSNK1G1). Validated for WB and ELISA. Recognizes cytoplasmic protein involved in DNA replication and repair. Supplied as liquid, unconjugated, affinity-purified, in PBS/glycerol buffer.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| ELISA | 1 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing amino acids 298–422 of human CSNK1G1 (NP_071331.2) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.86 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2914682 |
Product Specific Information
Positive test controls include Jurkat, HeLa, 293T, MCF7, and A-549 cell lines.
The target protein is typically localized in the cytoplasm.
Immunogen sequence:
FFEKPDYEYL RTLFTDLFEK KGYTFDYAYD WVGRPIPTPV GSVHVDSGAS AITRESHTHR DRPSQQQPLR NQVVSSTNGE LNVDDPTGAH SNAPITAHAE VEVVEEAKCC CFFKRKRKKT AQRHK
Target Information
Casein kinase I (CKI) and casein kinase II (CKII) are families of serine/threonine protein kinases found in all eukaryotes.
CKI family members (α, γ, ε, δ) regulate cytoplasmic and nuclear processes such as DNA replication and repair.
CKII typically forms a tetrameric complex (α2β2 or αα′β2), with the α catalytic subunit activated by the β regulatory subunit through autophosphorylation.
CKII phosphorylates over 100 substrates, including nuclear oncoproteins, transcription factors, and DNA metabolism enzymes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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