Thermo Fisher Scientific C. tetani TeNT Light Chain Monoclonal Antibody (604023)

Applications

Tested Dilution

Publications

Western Blot (WB)

2 µg/mL

-

Immunoprecipitation (IP)

25 µg/mL

-

Product Specifications

Species Reactivity

Bacteria

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

604023

Immunogen

E. coli-derived recombinant C. tetani TeNT Light Chain Pro2-Gly430

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.5 mg/mL

Purification

Protein A/G

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2610622

Product Specific Information

In direct ELISAs and Western blots, no cross-reactivity with recombinant TeNT Heavy Chain is observed.

Reconstitute in sterile PBS to a final concentration of 0.5 mg/mL.

Target Information

Tetanus toxin, a member of the peptidase M27 family of proteins, is produced by the anaerobic spore-forming bacteria Clostridium tetani as a single-chain polypeptide that is 1315 amino acids (aa) in length. The protein is subsequently cleaved by an endogenous protease to yield the 52 kDa tetanus toxin light chain (TeNT-LC), which is 457 aa in length, and the 98 kDa tetanus toxin heavy chain that is 858 aa long. The light and heavy chains are linked by a disulfide bridge and are non-toxic after separation. Tetanus toxin acts by inhibiting neurotransmitter release. It binds to peripheral neural synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons. It inhibits neurotransmitter release by acting as a zinc endopeptidase that catalyzes the hydrolysis of the 76-Gln- -Phe-77 bond of synaptobrevin-2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.