Thermo Fisher Scientific C. tetani TeNT Light Chain Monoclonal Antibody (604023)
C. tetani TeNT Light Chain을 인식하는 Mouse monoclonal antibody. Western blot 및 Immunoprecipitation에 적합. E. coli 유래 항원으로 제작되었으며, 비결합(Unconjugated) 형태로 제공. 단백질 A/G 정제, PBS + 5% trehalose 보존, -20°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 2 µg/mL |
| Immunoprecipitation (IP) | 25 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bacteria |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 604023 |
| Immunogen | E. coli-derived recombinant C. tetani TeNT Light Chain (Pro2–Gly430) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.5 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS with 5% trehalose |
| Contains | No Preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2610622 |
Product Specific Information
- In direct ELISAs and Western blots, no cross-reactivity with recombinant TeNT Heavy Chain is observed.
- Reconstitute in sterile PBS to a final concentration of 0.5 mg/mL.
Target Information
Tetanus toxin, a member of the peptidase M27 family, is produced by the anaerobic spore-forming bacterium Clostridium tetani as a single-chain polypeptide of 1315 amino acids. It is cleaved by an endogenous protease into:
- 52 kDa light chain (TeNT-LC, 457 aa)
- 98 kDa heavy chain (858 aa)
These chains are linked by a disulfide bridge and are non-toxic when separated.
Tetanus toxin inhibits neurotransmitter release by acting as a zinc endopeptidase that hydrolyzes the 76-Gln–Phe-77 bond of synaptobrevin-2. It binds to peripheral neural synapses, is internalized, and transported retrogradely into the spinal cord, where it interferes with neuronal communication.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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