Thermo Scientific™

RevertAid™ Master Mix

Thermo Scientific RevertAid Master Mix is a complete system for efficient synthesis of first-strand cDNA from mRNA or total RNA자세히 알아보기

Thermo Scientific RevertAid Master Mix is a complete system for efficient synthesis of first-strand cDNA from mRNA or total RNA templates. The mix includes RevertAid Reverse Transcriptase (RT), which has lower RNase H activity compared to AMV reverse transcriptase. RevertAid Master Mix provides all cDNA synthesis reaction components in a convenient two-tube master mix. It is optimized for highly efficient cDNA synthesis for two-step quantitative RT-PCR (RT-qPCR) applications. The two-tube formulation has a shorter reaction time and requires fewer pipetting steps than the RevertAid RT Reverse Transcription Kit, while maintaining high performance.

Highlights
• Fast—short reaction time (typically 15–30 min)
• Convenient—easy workflow with few pipetting steps
• Reliable—reverse transcription of both abundant and limited targets
• Flexible—optimized 2-step protocol, enabling multiple PCR reactions from a single reverse-transcription reaction

Applications
First strand cDNA synthesis for RT-PCR and RT-qPCR
• Construction of full length cDNA libraries
• Antisense RNA synthesis

사양

형식Master Mix

반응 속도30 min.

기술Reverse Transcription

수량50 reactions

역전사 효소RevertAid

리보뉴클레아제 H 활성Yes

배송 조건Dry Ice

용도(애플리케이션)Real Time PCR (qPCR)

최종 제품 유형First-Strand cDNA

반응 수50 Reactions

반응 형식Premixed Components

시약 유형Reverse Transcription

시작 물질RNA

Unit SizeEach