Thermo Fisher Scientific COX2 Monoclonal Antibody (COX 229), Alexa Fluor 647, eBioscience

Applications

• Flow Cytometry (Flow): 0.25 µg/test

Product Specifications

Additional Formats

• Alexa Fluor 488
• eFluor 450
• PE
• PE-Cyanine7
• Request custom conjugation

Product Specific Information

This COX 229 monoclonal antibody recognizes human COX2 (PTGS2) and has been reported to cross-react with mouse and rat COX2. For optimal performance, use with the Foxp3 / Transcription Factor Staining Buffer Set and protocol.

• Applications Reported: Intracellular staining followed by flow cytometric analysis.
• Applications Tested: Tested by intracellular staining of stimulated normal human peripheral blood cells using the Foxp3/Transcription Factor Staining Buffer Set (Product #00-5523-00).

Recommended Usage: ≤0.25 µg per test (100 µL final volume). Cell number may range from 10⁵–10⁸ cells/test. Optimize titration for best performance.

Fluorescence Properties:
Excitation: 633–647 nm
Emission: 668 nm
Laser: Red Laser

Target Information

COX2 converts arachidonate to prostaglandin H2 (PGH2), a key step in prostanoid synthesis including inflammatory prostaglandins. It is constitutively expressed in endothelium, kidney, and brain, and up-regulated in cancer and inflammation. COX2 up-regulation is linked to increased cell adhesion, resistance to apoptosis, and tumor angiogenesis. It is inhibited by calcitriol (vitamin D) and targeted by NSAIDs such as aspirin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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