
Thermo Fisher Scientific Goat anti-Human IgA Cross-Adsorbed Secondary Antibody, HRP
HRP 결합 염소 항-인간 IgA 2차 항체로 Western blot, IHC, ELISA에 적합합니다. 교차흡착 처리로 비특이적 결합 최소화. 친화 크로마토그래피로 정제되어 높은 특이성과 재현성을 제공합니다. 연구용으로만 사용.
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Thermo Fisher Scientific Goat anti-Human IgA Cross-Adsorbed Secondary Antibody, HRP
Applications 및 희석 배수
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:20,000 |
| Immunohistochemistry (IHC) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Human IgA |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.1% ProClin 150 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2535564 |
| Target | IgA |
| Cross Adsorption | Against bovine, mouse and rabbit serum proteins |
| Antibody Form | Whole Antibody |
Additional Formats
- [AP conjugate]
- [Biotin conjugate]
- [FITC conjugate]
- [TRITC conjugate]
- [Request custom conjugation]
Product Specific Information
Rehydrate with 1.1 mL of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Store lyophilized material at 2–8°C. For long-term storage after reconstitution, dilute with 50% glycerol and store at -20°C as a liquid.
Based on immunoelectrophoresis, no reactivity is observed to non-immunoglobulin human serum immunoglobulins, light chains on all human immunoglobulins, or serum proteins from bovine, mouse, or rabbit.
Target Information
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins. They are useful in detection, sorting, or purification of specific targets.
Secondary antibodies offer increased versatility, enabling use of many detection systems (e.g., HRP, AP, fluorescence). They provide greater sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
Most commonly, secondary antibodies are generated by immunizing the host animal with pooled immunoglobulins from the target species, followed by purification and modification (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to produce highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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