Thermo Fisher Scientific HMGB1 Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Target Information

HMGB1 (High-mobility group box-1) protein was originally described as a nuclear non-histone DNA-binding chromosomal protein. Recent studies show that damaged or necrotic cells release HMGB1 into the extracellular space, where it functions as a proinflammatory cytokine.

Mouse HMGB1 is a 215 amino acid single-chain polypeptide with three domains:

• Two tandem-linked positively charged DNA-binding domains (HMG box A: aa 9–79; box B: aa 89–162)
• A negatively charged 30 amino acid C-terminal acidic tail region

Residues 28–44 and 180–185 contain nuclear localization signals (NLS).
The cytokine activity resides in the B box, while the A box associates with transcription factor helix-loop-helix domains.

HMGB1 acts as:

• An inflammatory mediator promoting monocyte migration and cytokine secretion
• A mediator of T cell–dendritic cell interaction

It signals through receptors such as RAGE, TLR2, and TLR4. HMGB1 is highly conserved and found in nuclei and cytoplasm of most cell types, serving as a key mediator of inflammation in both sterile and infection-associated responses.
Elevated HMGB1 levels are observed in sterile tissue injury, infection, sepsis, arthritis, and ischemia-reperfusion injury.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.