Thermo Fisher Scientific NEK9 Polyclonal Antibody
NEK9 단백질을 인식하는 Thermo Fisher Scientific의 토끼 폴리클로날 항체. IHC(P)에서 사용 가능하며, 인간 및 생쥐 시료에 반응. 항원 친화 크로마토그래피로 정제되었으며, PBS(glycerol 포함) 용액 형태로 제공. 연구용으로만 사용.
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Applications
- Immunohistochemistry (Paraffin) (IHC (P)): 1:25–1:100
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse, Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to residues near the C-terminal of human NIMA-related kinase 9 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 40% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2636068 |
Product Specific Information
The antibody detects endogenous levels of total NEK9 protein.
Target Information
NEK9, a NEK type protein kinase, regulates chromosome alignment and segregation in mitosis. The protein has:
- An N-terminal NIMA-like catalytic domain
- A central domain with homology to the guanine nucleotide exchange factor for the GTPase Ran (RCC1)
- A C-terminal coiled-coil domain
It is phosphorylated by active p34(Cdc2) and is capable of autophosphorylation and oligomerization. The activated protein binds Nek6 and RCC1, and associates with the human homolog of the Drosophila protein Bicaudal D (Bicd2) in vivo, phosphorylating this coiled-coil protein in vitro. NEK9 expression has been documented in normal human brain. ESTs have been isolated from several tissue libraries, including normal bone, lymph, thymus, and cancerous cervix, eye, lymph, and placenta.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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