Thermo Fisher Scientific MRPL42 Monoclonal Antibody (OTI1A4), TrueMAB
인간 MRPL42 단백질을 인식하는 단클론 항체로 Western blot 및 IHC(P) 검증 완료. 동결건조 형태로 제공되며, PBS 버퍼와 트레할로오스 포함. 연구용으로 단백질 합성 관련 미토콘드리아 단백질 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1A4 |
| Immunogen | Full length human recombinant protein of human MRPL42 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
MRPL42 is a mammalian mitochondrial ribosomal protein encoded by nuclear genes, involved in mitochondrial protein synthesis.
Mitoribosomes consist of a small 28S and a large 39S subunit, with approximately 75% protein to rRNA composition, unlike prokaryotic ribosomes.
Mammalian mitoribosomes lack the 5S rRNA found in prokaryotic ribosomes.
The MRPL42 gene product is associated with both 28S and 39S subunits, with multiple transcript variants due to alternative splicing.
Pseudogenes are located on chromosomes 4q, 6p, 6q, 7p, and 15q.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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