Thermo Fisher Scientific cKit Polyclonal Antibody
Western blot, ELISA, IP 등 다양한 응용에 적합한 Rabbit Polyclonal c-Kit 항체. Human, Dog, Pig 등 여러 종에 반응하며, 합성 펩타이드(aa 950–976)로 면역화. Affinity chromatography로 정제된 액상 항체로, -20°C에서 보관.
- 카탈로그번호
- CKIT-131AP
- 판매단위
- pk
카탈로그
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1:50–1:250 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Human, Non-human primate, Pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide within amino acid region 950–976 on human Tyrosine-Kinase c-Kit protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5–1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer (pH 7.4–7.8) with 0.5% BSA, 30% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | –20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
KIT (c-KIT) is a proto-oncogene and a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). KIT was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. KIT and its ligand regulate the growth and activation of various hematopoietic and non-hematopoietic cells.
Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Deregulation of the KIT receptor tyrosine kinase (e.g., D816V mutation) is a therapeutic target in neoplastic mast cell growth.
c-Kit is expressed in hematopoietic stem cells, germ cells, mast cells, and gastrointestinal tract cajal cells. Upon binding of its ligand SCF (stem cell factor), c-Kit dimerizes, leading to receptor activation and autophosphorylation of tyrosine residues (e.g., Tyr703). This allows docking of Grb2 and activation of the Ras/ERK pathway. SCF/c-Kit also activates PI3K, PLC-γ, and JAK/STAT pathways, essential for hematopoiesis, stem cell maintenance, and gametogenesis.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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