Thermo Fisher Scientific PECAM1 Monoclonal Antibody (OTI3H2), TrueMAB
PECAM1(CD31) 단백질을 인식하는 mouse monoclonal antibody로, WB, IHC, ICC, Flow Cytometry 등 다양한 응용 가능. 고순도 affinity chromatography로 정제된 액상 형태이며, 인체 PECAM1 full-length recombinant protein을 면역원으로 사용. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:150 |
| Flow Cytometry (Flow) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3H2 |
| Immunogen | Full-length human recombinant protein of human PECAM1 produced in HEK293T cells (Target Family: CD31 (PECAM-1), UniProt ID: P16284-1, Antigen Range: 1–738) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling through immunoreceptor tyrosine-based inhibitory motifs (ITIMs). These motifs, upon phosphorylation by associated kinases, serve as docking sites for protein-tyrosine phosphatases. CD31 is expressed ubiquitously within the vascular compartment and mainly located at junctions between adjacent cells. The N-terminal Ig-like domain mediates homophilic binding, important for cell-cell interactions. CD31 plays roles in integrin-mediated adhesion, transendothelial migration, angiogenesis, apoptosis, immune regulation, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis, and thrombosis. It is a key regulatory molecule in the vascular system.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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