Thermo Fisher Scientific Estrogen Receptor alpha Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

PA1-309 detects estrogen receptor (ER) alpha from human, porcine, and rat tissues and cells.
It has been successfully used in Western blot, immunofluorescence, and immunohistochemistry procedures.
By Western blot, this antibody detects an ~64 kDa protein representing ER alpha from MCF-7 cell extracts.
Immunohistochemical staining of ER alpha in rat prostate with PA1-309 results in primarily nuclear staining.

The immunogen is a synthetic peptide corresponding to residues N(21) E L E P L N R P Q L K(32) C of human ER alpha.
This sequence is conserved among human, rat, mouse, rabbit, sheep, porcine, and bovine species.
PA1-309 immunizing peptide (Cat. # PEP-037) is available for neutralization and control experiments.
Recombinant ER alpha expressed in baculovirus system (Cat. # RP-310) can be purchased for control experiments in Western blot and gel shift assays.

Target Information

Estrogen Receptors (ER) are nuclear receptors belonging to the steroid/thyroid hormone receptor superfamily.
They function as ligand-activated transcription factors that bind estrogen, inducing dimerization and interaction with estrogen response elements (EREs) in DNA.
ERs regulate gene transcription through coactivator or corepressor recruitment.
Two forms exist—ER alpha and ER beta—encoded by ESR1 and ESR2 genes.
Alternative splicing produces at least four ER alpha isoforms (66, 53, 47, and 35 kDa).
ERs are widely expressed and play essential roles in sexual development, reproduction, and bone health.
They are implicated in diseases such as breast cancer, endometrial cancer, and osteoporosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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