Thermo Fisher Scientific PCGF5 Polyclonal Antibody
Rabbit polyclonal antibody against human PCGF5. Validated for IHC(P) and ICC/IF. Recognizes recombinant PCGF5 protein. Suitable for chromatin remodeling and gene regulation studies. For research use only.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:500 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human PCGF5. Recombinant protein control fragment (Product # RP-97187). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2645280 |
Product Specific Information
Immunogen sequence:
TCIVQHFEDS NDCPRCGNQV HETNPLEMLR LDNTLEEIIF KLVPGLREQE LERESEFWKK NKPQENGQDD TSKAD
Sequence identity of immunogen to orthologs:
- Mouse: 95%
- Rat: 93%
Target Information
Component of a Polycomb group (PcG) multiprotein PRC1-like complex, which maintains the transcriptionally repressive state of many genes, including Hox genes, throughout development.
The PcG PRC1 complex acts via chromatin remodeling and histone modification, mediating monoubiquitination of histone H2A at Lys-119, resulting in heritable changes in gene expression (PubMed:26151332).
Within the PRC1-like complex, PCGF5 regulates RNF2 ubiquitin ligase activity and functions redundantly with PCGF3 in mediating histone H2A Lys-119 monoubiquitination on the X chromosome, essential for normal X-chromosome inactivation in females.
[UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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