Applied Biosystems™

AmpFLSTR™ Yfiler™ PCR Amplification Kit

The AmpFLSTR Yfiler PCR Amplification Kit permits the analysis of complex male:female DNA mixtures that often yield ambiguous or inconclusive자세히 알아보기

The AmpFLSTR Yfiler PCR Amplification Kit permits the analysis of complex male:female DNA mixtures that often yield ambiguous or inconclusive results with autosomal STRs.

Key Features of the AmpFLSTR Yfiler PCR Amplification Kit:

• Analyze trace quantities of male DNA that may be present in a large background of female DNA
• Combine the inherent male specificity of Y-STRs with the robust performance of an AmpFLSTR kit
• Amplify 17 Y-STR loci in a single PCR reaction which yields the highest discriminatory capacity of any commercially available Y-STR kit
• Search the user-friendly on-line haplotype database to easily determine the frequency of any Yfiler kit haplotype
• Use this powerful tool for the analysis of cases that contain a suspect(s)

Combines the Inherent Male Specificity of Y-STRs with the Robust Performance of an AmpFLSTR Kit
Mixtures of body fluids and/or tissues from a male and female are frequently encountered with sexual assault casework and other violent crimes. These mixed samples often contain trace quantities of male DNA present in a large background of female DNA. Typical samples can include vaginal swabs containing a small number of sperm cells, body swabs from female victims of oral assaults, and fingernail scrapings/clippings from female victims of violent assaults. Standard autosomal STR analysis may be unable to detect the minor male contributor in these types of samples, while the Yfiler kit can routinely detect a minor male contributor present at less than 1:1000.

Provides the Highest Discriminatory Capacity of Any Commercially Available Y-STR Kit
The AmpFLSTR Yfiler PCR Amplification Kit amplifies 17 Y-STR loci in a single PCR reaction using 5-dye chemistry to provide the highest discriminatory capacity of any commercially available Y-STR kit. The Yfiler kit can distinguish more than 98% of all unrelated males from one another, and therefore offers the discriminatory power necessary to conduct meaningful forensic DNA analysis.

A Powerful Tool for Suspect Casework
The Yfiler kit is a powerful tool for the analysis of cases that contain a suspect(s). A significant proportion of DNA cases contain a suspect who has been identified by conventional law enforcement techniques. For these cases, the Yfiler kit is a powerful tool that can be used to include or exclude a suspect(s) associated with an investigation.

Complete Validated Solution
Applied Biosystems offers a complete solution for your Human Identification needs. Our system includes DNA quantification kits, a range of AmpFLSTR kits, instrumentation, software, and consumables. The Yfiler kit is part of the most comprehensively validated systems for STR-based Human Identification. All reagents are developed in accordance with guidelines from leading worldwide forensic organizations such as SWGDAM and DAB.

For licensing and limited use restrictions visit thermofisher.com/HIDlicensing.

사양

앰플리콘 크기<340 bp

검출 방법qPCR

GC-Rich PCR PerformanceHigh

중합효소AmpliTaq Gold DNA Polymerase

반응 속도Standard

기술STR (Short Tandem Repeat) Analysis

용도(장비)310 Genetic Analyzer, 3100-Avant Genetic Analyzer, 3130xl Genetic Analyzer, GeneAmp 9600, GeneAmp 9700, 3130 Genetic Analyzer, 3730 DNA Analyzer, 3500xL Genetic Analyzer, 3500 Genetic Analyzer

라벨 또는 염료FAM, LIZ, NED, PET, VIC

매트릭스 및 크기 표준GeneScan 500 LIZ Size Standard, Matrix Standard Set DS-33

제품라인AmpFLSTR, Yfiler

제품 유형PCR Amplification Kit

수량100 reactions

배송 조건Dry Ice

타겟 위치DYS438, DYS393, DYS385a⁄b, DYS389I⁄II, DYS458, DYS437, DYS391, DYS392, DYS635 (Y GATA C4), Y GATA H4, DYS19, DYS390, DYS439, DYS456, DYS448

반응 형식SuperMix or Master Mix

샘플 종류DNA (Genomic), Forensic Sample

부피25 μL/Reaction

Unit SizeEach