Thermo Fisher Scientific Viral Protein U Polyclonal Antibody, FITC
FITC 결합된 Viral Protein U 폴리클로날 항체로 다양한 면역분석(WB, IHC, ICC, ELISA 등)에 사용 가능. Rabbit IgG 기반으로 고순도 친화 크로마토그래피 정제. 바이러스 단백질 VPU 인식, 형광 검출에 적합. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (IHC) | 1:50–1:250 |
| Immunocytochemistry (ICC/IF) | 1:50–1:250 |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1:50–1:250 |
| Immunomicroscopy (IM) | 1:50–1:200 |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Virus |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide within amino acid region 50–90 on VPU peptide |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5–1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer, pH 7.4–7.8, with 0.5% BSA, 30% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | −20°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Viral Protein U (Vpu) enhances virion budding by targeting host CD4 and Tetherin/BST2 for proteasomal degradation.
Degradation of CD4 prevents premature interaction between viral Env and host CD4 in the endoplasmic reticulum.
Degradation of Tetherin/BST2 is essential for virion release, as BST2 tethers new viral particles to the host membrane.
Vpu bridges CD4 or BST2 to BTRC, a substrate recognition subunit of the Skp1/Cullin/F-box E3 ubiquitin ligase, inducing ubiquitination and degradation.
Altered E3 ligase specificity by Vpu may promote degradation of host IKBKB, down-regulating NF-κB and inducing apoptosis.
Ion channel activity has been suggested, though its in vivo role remains uncertain.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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