Thermo Fisher Scientific KEAP1 Monoclonal Antibody (OTI1B4), TrueMAB
KEAP1 단백질을 인식하는 Mouse IgG1 단클론 항체로, Western blot, IHC, Flow cytometry에 적합합니다. 인간 및 생쥐 반응성, 고순도 친화 크로마토그래피 정제, 동결건조 형태로 안정적 보관이 가능합니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1B4 |
| Immunogen | Full length human recombinant protein of human KEAP1 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
KEAP1 (Kelch-like ECH-associated protein 1) contains KELCH-1-like and BTB/POZ domains.
It interacts with NF-E2-related factor 2 (Nrf2) in a redox-sensitive manner.
Upon dissociation, Nrf2 translocates to the nucleus, inducing expression of the catalytic subunit of gamma-glutamylcysteine synthetase.
Two alternatively spliced transcript variants encoding the same isoform have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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