Thermo Fisher Scientific IDH3G Polyclonal Antibody
Human, Mouse, Rat에서 반응하는 Rabbit Polyclonal 항체로, Western blot, IHC(P), ICC/IF에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, 0.1 mg/mL 농도의 액상 형태. IDH3G 단백질을 인식하며 연구용으로 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:500 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human IDH3G. Recombinant protein control fragment (Product #RP-91985). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C and avoid freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2642624 |
Product Specific Information
Immunogen sequence:
FDVMVMPNLY GNIVNNVCAG LVGGPGLVAG ANYGHVYAVF ETATRNTGKS IANKNIANPT ATLLASCMML DHLKLHSYAT SIRKAVLASM DNENMHTPDI GGQGTTSEAI QDVI
Antigen sequence identity:
- Mouse: 98%
- Rat: 98%
Target Information
Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes are divided into two subclasses: NAD(+)-dependent and NADP(+)-dependent.
Among the five known isocitrate dehydrogenases, three are NAD(+)-dependent and located in the mitochondrial matrix, while two are NADP(+)-dependent (one mitochondrial and one cytosolic).
NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer composed of two alpha, one beta, and one gamma subunit.
The IDH3G gene encodes the gamma subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase and is a candidate gene for periventricular heterotopia. Several alternatively spliced transcript variants have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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