Thermo Fisher Scientific HARS Polyclonal Antibody
HARS 단백질을 인식하는 Rabbit Polyclonal Antibody로, Western blot, IHC, ICC/IF에 사용 가능. Human, Mouse, Rat 반응성. 항원 친화 크로마토그래피로 정제되었으며, PBS/glycerol buffer에 보관. 연구용으로 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:1,000 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human HARS. Recombinant protein control fragment (Product #RP-96931) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_2642310 |
Product Specific Information
Immunogen sequence:
MAERAALEEL VKLQGERVRG LKQQKASAEL IEEEVAKLLK LKAQLGPDES KQKFVLKTPK GTRD
Highest antigen sequence identity to orthologs:
- Mouse: 86%
- Rat: 86%
Target Information
Aminoacyl-tRNA synthetases are enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a cytoplasmic enzyme belonging to the class II family of aminoacyl-tRNA synthetases. It synthesizes histidyl-transfer RNA, essential for incorporating histidine into proteins. The gene is located in a head-to-head orientation with HARSL on chromosome 5, sharing a bidirectional promoter. This gene product is a frequent target of autoantibodies in polymyositis/dermatomyositis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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