Thermo Fisher Scientific Phospho-KSR1 (Ser392) Recombinant Rabbit Monoclonal Antibody (KSR1S392-3A4)
인간 및 마우스 반응성의 recombinant rabbit monoclonal antibody로, Ser392 인산화 KSR1 단백질 검출에 사용됩니다. 높은 특이성과 일관된 로트 간 재현성을 제공합니다. Flow Cytometry에 최적화되어 있으며, 단백질 A/G 정제 및 -20°C 보관 조건을 가집니다.
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Applications
Flow Cytometry (Flow)
- Tested Dilution: 1–1,000 ng/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | KSR1S392-3A4 |
| Immunogen | A synthetic phospho-peptide corresponding to residues surrounding Ser392 of human phospho KSR1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol, 0.1% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2896951 |
Additional Conjugate Formats
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems clone specific antibody DNA sequences from immunoreactive rabbits, followed by screening to select the best production candidates.
Advantages include:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to the larger rabbit immune repertoire
Target Information
KSR1 is part of a multiprotein signaling complex that promotes phosphorylation of Raf family members and activation of downstream MAP kinases. Independently of its kinase activity, it acts as a MAP2K1/MEK1 and MAP2K2/MEK2-dependent allosteric activator of BRAF. Upon binding to MAP2K1/MEK1 or MAP2K2/MEK2, KSR1 dimerizes with BRAF and promotes BRAF-mediated phosphorylation of MAP2K1/MEK1 and/or MAP2K2/MEK2 (PubMed:29433126). It promotes activation of MAPK1 and/or MAPK3 in response to EGF and cAMP. The physiological relevance of its kinase activity remains uncertain. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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