Thermo Fisher Scientific Pan Cytokeratin Monoclonal Antibody (AE1/AE3), eFluor 615, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Alexa Fluor 488
• eFluor 570
• eFluor 660
• Request custom conjugation

Product Specific Information

The monoclonal antibodies AE1 and AE3 recognize many acidic and basic cytokeratin family members. Cytokeratins are intermediate filament proteins forming part of the cytoskeleton. They are expressed in epithelial cells and are developmentally regulated. Many tumors also express cytokeratins, aiding in identifying the origin of neoplasms.

• AE3 antibody recognizes 65–67 triplet, 64, 59, 58, 56, 54, and 52 kDa proteins (CK1–8).
• AE1 antibody recognizes 56.5, 54, 50, 48, and 40 kDa proteins (CK10, 14, 15, 16, 19).
• Applicable to tissue from mouse, human, rat, primates, dog, cat, rabbit, and chicken.

Applications Reported:
Used for immunohistochemical staining, immunocytochemistry, frozen tissue sections, and formalin-fixed paraffin-embedded sections.

Applications Tested:
Tested by immunocytochemistry on methanol-fixed MCF7 cells at ≤5 µg/mL. Recommended to titrate for optimal performance. Not validated for flow cytometry.

Filter Recommendation:
Use a filter capturing 615 nm emission (e.g., Excitation 560/55, 585LP, Emission 645/75). Alexa Fluor™ 594 filter is acceptable.

Excitation/Emission: 595 nm / 615 nm
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Cytokeratin pan represents a subfamily of intermediate filament proteins with biochemical diversity, found in human epithelial tissues with at least 20 polypeptides (40–68 kDa, pI 4.9–7.8). Expression patterns vary by epithelial type and differentiation stage. Cytokeratin profiles assist in distinguishing epithelial malignancies and are valuable in tumor diagnosis, cytopathology, and cancer research.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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