Thermo Fisher Scientific Pan Cytokeratin Monoclonal Antibody (AE1/AE3), eFluor 615, eBioscience
Pan Cytokeratin 단백질을 인식하는 AE1/AE3 클론의 마우스 단일클론 항체. eFluor 615 형광 표지로 면역형광, IHC, ICC 등에 사용 가능. 다양한 종(인간, 마우스, 개 등)에 반응하며, 세포골격 연구 및 종양 기원 분석에 유용.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 1 publication |
| Immunohistochemistry (IHC) | - | View 9 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-Dependent | - |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent | View 1 publication |
| Immunocytochemistry (ICC/IF) | 5 µg/mL | View 3 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Human, Mouse, Non-human primate, Rabbit, Rhesus monkey |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | AE1/AE3 |
| Conjugate | eFluor™ 615 |
| Excitation / Emission Max | 595 / 614 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10804663 |
Available Formats
Product Specific Information
The monoclonal antibodies AE1 and AE3 recognize many acidic and basic cytokeratin family members. Cytokeratins are intermediate filament proteins forming part of the cytoskeleton. They are expressed in epithelial cells and are developmentally regulated. Many tumors also express cytokeratins, aiding in identifying the origin of neoplasms.
- AE3 antibody recognizes 65–67 triplet, 64, 59, 58, 56, 54, and 52 kDa proteins (CK1–8).
- AE1 antibody recognizes 56.5, 54, 50, 48, and 40 kDa proteins (CK10, 14, 15, 16, 19).
- Applicable to tissue from mouse, human, rat, primates, dog, cat, rabbit, and chicken.
Applications Reported:
Used for immunohistochemical staining, immunocytochemistry, frozen tissue sections, and formalin-fixed paraffin-embedded sections.
Applications Tested:
Tested by immunocytochemistry on methanol-fixed MCF7 cells at ≤5 µg/mL. Recommended to titrate for optimal performance. Not validated for flow cytometry.
Filter Recommendation:
Use a filter capturing 615 nm emission (e.g., Excitation 560/55, 585LP, Emission 645/75). Alexa Fluor™ 594 filter is acceptable.
Excitation/Emission: 595 nm / 615 nm
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Cytokeratin pan represents a subfamily of intermediate filament proteins with biochemical diversity, found in human epithelial tissues with at least 20 polypeptides (40–68 kDa, pI 4.9–7.8). Expression patterns vary by epithelial type and differentiation stage. Cytokeratin profiles assist in distinguishing epithelial malignancies and are valuable in tumor diagnosis, cytopathology, and cancer research.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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