Thermo Fisher Scientific CD25 Monoclonal Antibody (PC61 5.3), Alexa Fluor 488

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

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View 1 publication 1 publication

Flow Cytometry (Flow)

0.1-0.25 µg / 1x10^6 cells

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Product Specifications

Published species

Not Applicable

Host/Isotype

Rat / IgG1

Class

Monoclonal

Type

Antibody

Clone

PC61 5.3

Immunogen

B6.1 cells.

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Storage conditions

4° C

Shipping conditions

Wet ice

RRID

AB_10373683

Target Information

CD25 (IL2 receptor alpha chain/IL2RA) is a cytokine that plays a role in the proliferation of T and B lymphocytes. The receptor of this cytokine (IL2RA) is a heterotrimeric protein complex with a gamma chain also shared by interleukin 4 (IL4) and interleukin 7 (IL7). IL2RA, IL2R beta chain (IL2RB), and the IL2R gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric IL2RA chains result in low-affinity receptor, while homodimeric IL2RB chains produce a medium-affinity receptor. The expression of IL2 in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. IL2 is primarily produced by mature T cells. IL2 plays an important role as a growth factor, differentiation factor, and regulator of cell death. IL-2 stimulates the proliferation of B cells, augments natural killer cell activity, and inhibits granulocyte macrophage colony formation. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests a role in the immune response to antigenic stimuli. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.