Thermo Fisher Scientific Mouse Hematopoietic Lineage Antibody Cocktail, eFluor 450, eBioscience

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Flow Cytometry (Flow)

20 µL/test

View 26 publications 26 publications

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat

Class

Cocktail

Type

Antibody

Conjugate

eFluor™ 450 eFluor™ 450 eFluor™ 450

Excitation/Emission Max

405/445 nm View spectra

Form

Liquid

Concentration

20 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10426799

Product Specific Information

Description: The Mouse Hematopoietic Lineage eFluor™ 450 Cocktails contains eFluor™ 450-conjugated antibodies that can be used to identify, enrich and/or deplete cells committed to the T, B, NK, myeloid and erythroid lineages based on the expression of cell surface antigens.

This cocktail contains the following antibodies:

Anti-Mouse CD3 (17A2) eFluor™ 450

Anti-Mouse CD45R (B220) (RA3-6B2) eFluor™ 450

Anti-Mouse CD11b (M1/70) eFluor™ 450

Anti-Mouse TER-119 (TER-119) eFluor™ 450

Anti-Mouse Ly-G6 (Gr-1) (RB6-8C5) eFluor™ 450

Applications Reported: The Mouse Hematopoietic Lineage eFluor™ 450 Cocktail has been reported for use in flow cytometric analysis.

Applications Tested: The Mouse Hematopoietic Lineage eFluor™ 450 Cocktail has been pre-titrated and tested by flow cytometric analysis of C57Bl/6 bone marrow cells. This can be used at 20 µL per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

eFluor™ 450 is an alternative to Pacific Blue™. eFluor™ 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Target Information

Hematopoietic Lineage antibodies can be used to identify, enrich and/or deplete cells committed to the T, B, NK, myeloid and erythroid lineages based on their expression of cell surface antigens.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.