Thermo Fisher Scientific M-CSF Monoclonal Antibody (131621)

Applications

ELISA (ELISA)

• Tested Dilution: 0.1–0.4 µg/mL (Detection), 2–8 µg/mL (Capture)

Neutralization (Neu)

• Tested Dilution: 0.3–1.5 µg/mL

Product Specifications

Product Specific Information

• In sandwich ELISAs, no cross-reactivity with recombinant human M-CSF, recombinant mouse SCF, Flt-3 Ligand, G-CSF, and GM-CSF is observed.
• Reconstitute at 0.5 mg/mL in sterile PBS.
• Endotoxin level is <0.10 EU per 1 µg of antibody (LAL method).

Target Information

M-CSF (Macrophage colony-stimulating factor, CSF-1) is a survival factor essential for the proliferation and development of monocytes, macrophages, and osteoclast progenitor cells.
M-CSF induces VEGF secretion by macrophages, promoting endothelial progenitor cell mobilization and neovascularization.
It exists as multiple bioactive isoforms differing in potency and stability. The full-length protein is membrane-spanning and can be expressed on the cell surface or cleaved in the secretory vesicle.
M-CSF forms a disulfide-bonded homodimer processed into glycoprotein or proteoglycan isoforms.
Binding to its receptor c-Fms (CSF-1R/CD115) triggers receptor dimerization, internalization, and degradation.
Functionally, M-CSF stimulates differentiation of hematopoietic stem cells into monocyte-macrophage populations.
Human M-CSF shows activity on mouse cells, but mouse CSF has no activity on human cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.