Thermo Fisher Scientific IRS1 Monoclonal Antibody (OTI3G10), TrueMAB
IRS1 단백질을 인식하는 Thermo Fisher Scientific의 단클론 항체로, Western blot 및 IHC(P)에서 검증됨. Human 시료에 반응하며, 농도 1 mg/mL의 액상 형태. Affinity chromatography로 정제되어 안정적인 신호 검출에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3G10 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1–280 of human IRS1 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
IRS-1 is a major substrate of the insulin receptor and becomes phosphorylated in response to stimulation by insulin, insulin-like growth factor 1 (IGF-1), and interleukin 4 (IL-4). It undergoes phosphorylation on serine, threonine, and tyrosine residues in various tissues. Casein kinase II mediates a portion of insulin-stimulated serine/threonine phosphorylation of IRS-1 in vivo, identifying Thr 502 and Ser 99 as key phosphorylation sites. These modifications are part of the intracellular insulin signaling cascade. IRS-1 also contains three putative 14-3-3 binding sites (Ser 270, Ser 374, Ser 641), with the Ser 270 motif located in the phosphotyrosine binding domain responsible for insulin receptor interaction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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배송/결제/교환/반품 안내
배송 정보
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