Thermo Fisher Scientific IFN alpha Monoclonal Antibody (EBI-1), eBioscience

Applications and Tested Dilutions

Product Specifications

Product Specific Information

Description:
Anti-Human IFN-alpha (EBI-1) inhibits the antiviral activity of interferon alpha. BMS160 specifically reacts with human recombinant and natural interferon alpha and can be used as a capture antibody in ELISA.

Applications Tested: ELISA, Functional Studies (Blocking)

Source: E. coli (amino acids Ser113–Ala271; accession # NP_000566)

Bioactivity:
The ED50, as measured by proliferation of mouse D10S cells, is ≤0.001 ng/mL, corresponding to a specific activity of ≥1.0 × 10⁹ Units/mg.

Endotoxin:
<0.1 ng/µg cytokine (LAL assay)

Purity:

98% (SDS-PAGE and HPLC)

Molecular Weight:
18 kDa

Storage and Handling:

• Quick spin vial prior to opening for best recovery.
• Use in sterile environment.
• Reconstitute in water to 0.1–1.0 mg/mL.
• Do not vortex.

Target Information

IFN alpha (Interferon alpha) is a Type I interferon belonging to a family of structurally related, species-specific proteins exclusive to vertebrates. Its interaction with receptor components activates signaling pathways involved in innate immune responses against viral infections.

In humans, the IFN alpha gene is located on chromosome 9. Type I interferons include 14 alpha isoforms and single beta, omega, epsilon, and kappa isoforms. Homologous molecules are found in many species, including mammals, birds, reptiles, and fish.

IFN alpha is primarily produced by plasmacytoid dendritic cells, plays a key role in antiviral innate immunity, and modulates adaptive immune responses. Dysregulation of IFN alpha is associated with diseases such as Hepatitis C and Hepatitis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.