Thermo Fisher Scientific IFN alpha Monoclonal Antibody (EBI-1), eBioscience

Applications

Tested Dilution

Publications

ELISA (ELISA)

Assay-Dependent

-

Neutralization (Neu)

-

View 1 publication 1 publication

Functional Assay (Functional)

Assay-Dependent

-

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

EBI-1

Immunogen

Rec. Human IFN alpha

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Ion-exchange chromatography, Ammonium sulfate precipitation

Storage buffer

PBS

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Wet ice

RRID

AB_10597749

Product Specific Information

Description: Anti-Human IFN-alpha (EBI-1) inhibits the antiviral activity of interferon alpha. BMS160 specifically reacts with human recombinant and natural interferon alpha and can be used as capture antibody in ELISA.

Applications Tested: ELISA, Functional Studies (Blocking).

Source: E. coli (amino acids Ser113-Ala271; accession # NP_000566).

Bioactivity: The ED50 of this protein, as measured by proliferation of mouse D10S cells, is less than or equal to 0.001 ng/mL, which corresponds to a specific activity of at least 1.0 x 10e9 Units/mg.

Endotoxin: Less than 0.1 ng/ug cytokine as determined by the LAL assay. Purity: >98% as determined by SDS-PAGE and HPLC.

Molecular Weight: 18 kDa.

Storage and handling: For best recovery, quick spin vial prior to opening. Use in sterile environment. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Target Information

IFN alpha (Interferon alpha) is a Type I interferon belonging to a family of structurally related, species-specific proteins exclusive to vertebrates. The interaction of IFN-alpha with its receptor component result in the activation of a number of signaling pathways which are mainly involved in innate immune response against viral infection. In humans, the gene for IFN alpha is located on on chromosome 9. The type I interferons consist of 14 different alpha isoforms (subtypes with slightly different specificities), and single beta, omega, epsilon and kappa isoforms. Homologous molecules are found in many species, including rats and mice (and most mammals), and have been identified in birds, reptiles and fish species. IFN alpha is produced primarily by plasmacytoid dendritic cells, is a potent component of the anti-viral innate immune response and modulates adaptive immunity. Diseases associated with the dysfunction of IFN alpha include Hepatitis C and Hepatitis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.