Thermo Fisher Scientific CD117 (c-Kit) Monoclonal Antibody (104D2), PE, eBioscience
인간 CD117(c-Kit)을 인식하는 104D2 클론의 단클론 항체로, PE 형광으로 표지되어 있습니다. Flow Cytometry 분석에 최적화되어 있으며, 혈액세포 및 비만세포의 증식·분화 연구에 활용됩니다. 4°C 암소 보관, 동결 금지.
- 카탈로그번호
- 12-1178-4x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD117 (c-Kit) Monoclonal Antibody (104D2), PE, eBioscience
Thermo Fisher Scientific CD117 (c-Kit) Monoclonal Antibody (104D2), PE, eBioscience™
Applications
- Flow Cytometry (Flow)
Tested Dilution: 5 µL (0.25 µg)/test
View 12 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 104D2 |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS (pH 7.2), with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2043798 |
Additional Formats
- APC
- APC-eFluor 780
- eFluor 450
- FITC
- PE-Cyanine5
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Super Bright 702
- Request custom conjugation
Product Specific Information
Description:
The 104D2 monoclonal antibody reacts with human CD117 (c-Kit), also known as Steel factor receptor and stem cell factor receptor. This 145 kDa tyrosine kinase receptor is expressed by hematopoietic progenitor cell subsets and mast cells. Interaction with Steel factor promotes proliferation and differentiation of hematopoietic progenitor cells and mast cell maturation. CD117 is also expressed by melanocytes and plays a role in signaling and activation.
Applications Reported:
Validated for flow cytometric analysis.
Applications Tested:
Pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. Recommended usage: 5 µL (0.25 µg) per test. A test is defined as the amount of antibody staining a cell sample in 100 µL final volume, with 10⁵–10⁸ cells/test.
Excitation / Emission:
488–561 nm / 578 nm; compatible with Blue, Green, and Yellow-Green lasers.
Filtration:
0.2 µm post-manufacturing filtered.
Target Information
KIT (c-KIT) is a proto-oncogene encoding a type III transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). It regulates growth and activation of hematopoietic and non-hematopoietic cells. Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Deregulation of KIT (e.g., mutation D816V) is a therapeutic target in neoplastic mast cell growth.
c-Kit is expressed in hematopoietic stem cells, germ cells, mast cells, and gastrointestinal tract Cajal cells. Upon SCF binding, c-Kit dimerizes, activating autophosphorylation of tyrosine residues (e.g., Tyr703), enabling Grb2 docking and Ras/ERK pathway activation. SCF/c-Kit signaling also activates PI3K, PLC-γ, and JAK/STAT pathways, essential for hematopoiesis, stem cell maintenance, and gametogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.