Thermo Fisher Scientific CF6-Neo Mouse Embryonic Fibroblasts, irradiated

Gibco™ CF6-Neo Mouse Embryonic Fibroblasts, Irradiated

Irradiated Gibco CF6-Neo Mouse Embryonic Fibroblasts (MEFs) are neomycin-resistant feeder cells that support healthy, undifferentiated human and mouse embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) in culture.
Manufactured by MTI-GlobalStem (equivalent to Cat. No. GSC-6005G), these MEFs are isolated from CF1 x C57BL/6 hybrid mice, mitotically arrested by irradiation, and tested to ensure performance and minimize contamination risk.
For mitomycin C-treated MEFs, see CF6-Neo Mouse Embryonic Fibroblasts, MitC-treated.

주요 특징

• 간편한 사용: 사전 비활성화된 즉시 사용 가능한 MEFs로 시간 절약
• 신뢰성 있는 배양: 세균, 진균, 마이코플라스마 및 병원체 테스트 완료
• 높은 생존율: 해동 후 생존율 95% 이상
• 네오마이신 내성: G418 내성 검증 완료, 약물 선택 실험에 적합

Ready-to-Use MEFs

Irradiated CF6-Neo MEFs save time by eliminating the need for animal facility work, dissections, and repeated cell expansions. Researchers can focus on experiments using these pre-inactivated, ready-to-use feeder cells.

Meticulously Tested Feeder Cells

Each lot is tested for bacteria, fungi, mycoplasma, and mouse pathogens to minimize contamination risk. Post-thaw viability typically exceeds 95%, and each lot is verified for its ability to support mouse and human ES cells.
These MEFs have also been used successfully with ESC/iPSC lines from other species but should be tested for compatibility in such cases.

Neomycin-Resistant Feeder Cells

CF6-Neo MEFs express neoR from a pPGKneobPa transgene, conferring high resistance to neomycin and geneticin (G418).
Each lot is tested for G418 tolerance, and survival thresholds are reported in the Certificate of Analysis (CoA) to assist in experimental design.
Use these feeder cells to maintain undifferentiated ESC or iPSC cultures during G418 selection.

제품 사양